Barley (Hordeum vulgare L.) is just about the world’s earliest domesticated and many important crop plants. It really is diploid with a large haploid genome of 5.1 gigabases (Gb). A new study on Barley has been published in the journal of Nature.
“USDA supports innovative genomics research that is really moving us forward to meeting the many challenges we face in food, fuel and agriculture production,” said Catherine Woteki, USDA’s Chief Scientist and Under Secretary for Research, Education, and Economics. “This important step toward full barley genome sequencing offers enormous potential for global food security. Using the tools of genetics and genomics, we are keeping farmers profitable and our food supply safe and abundant.”
Take a look at present a built-in and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a very structured whole-genome context. Researchers developed a physical map of 4.98 Gb, exceeding 3.90 Gb anchored with a high-resolution genetic map.
Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 ‘high-confidence’ genes with homology support from other plant genomes.
Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms a crucial regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data give a platform for both genome-assisted research and enabling contemporary crop improvement.
This study was supported by German Ministry of Education and Research (BMBF) grant 0314000 “BARLEX” to K.F.X.M., M.P., U.S. and N.S.; Leibniz Association grant (Pakt f. Forschung und Innovation) to N.S.; European project of the 7th framework programme “TriticeaeGenome” to R.W., A.S., K.F.X.M., M.M. and N.S.; SFB F3705, of the Austrian Wissenschaftsfond (FWF) to K.F.X.M.; ERA-NET PG project “BARCODE” grant to M.M., N.S. and R.W.; Scottish Government/BBSRC grant BB/100663X/1 to R.W., D.M., P.H., J.R., M.C. and P.K.; National Science Foundation grant DBI 0321756 “Coupling EST and Bacterial Artificial Chromosome Resources to Access the Barley Genome” and DBI-1062301 “Barcoding-Free Multiplexing: Leveraging Combinatorial Pooling for High-Throughput Sequencing” to T.J.C. and S.L.; USDA-CSREES-NRI grant 2006-55606-16722 “Barley Coordinated Agricultural Project: Leveraging Genomics, Genetics, and Breeding for Gene Discovery and Barley Improvement” to G.J.M., R.P.W., T.J.C. and S.L.; the Agriculture and Food Research Initiative Plant Genome, Genetics and Breeding Program of USDA-CSREES-NIFA grant 2009-65300-05645 “Advancing the Barley Genome” to T.J.C., S.L. and G.J.M.; BRAIN and NBRP-Japan grants to K.S., Japanese MAFF Grant (TRG1008)
Read full article here.
Klaus F. X. Mayer, Robbie Waugh, Nils Stein etal. “A physical, genetic and functional sequence assembly of the barley genome” doi:10.1038/nature11543
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